UAB Biosafety Manual

[Note: only sections of the manual that are currently available are offered for download]

    TABLE OF CONTENTS - 21 KBPDF file*

    Section 1.    Introduction - 14 KBPDF file*

    Section 2.    Program Administration - 53 KBPDF file*

      1. Office of the Vice President for Financial Affairs
      2. UAB Institutional Biosafety Committee
      3. The Department of Occupational Health & Safety
        1. Biosafety Division
        2. Biosafety Officer
      4. Deans/Department Chairs/Directors
      5. Principal Investigators/Laboratory Directors
      6. Laboratory Staff
      7. Liability Considerations
      8. Incidents of Non-compliance

    Section 3.    Project Registration

      1. New Project Registration
      2. Renewals
      3. Modifications of previously registered projects
      4. IBC/Biosafety Approval
      5. Appeal Process

    Section 4.    Principles of Biosafety - 33 KBPDF file*

      1. Containment

    4.1.1    Primary Containment (Safety Equipment)
    4.1.2    Secondary Containment (Facility Design)

      1. Basic Laboratory Safety
      2. Biosafety Levels – in brief

    Section 5.    Biohazards and Potentially Infectious Materials

      1. Definitions
      2. Potentially Infectious Materials Use
        1. Microbes
        2. Human Blood, Blood Products, Body Fluids and Tissues
        3. Tissue/Cell Culture

    5.3  Animal Use
    5.3.1   Minimum Biosafety Guidelines
    5.3.2   Infected Animals
    5.3.3   Use of Non-human Primates
    5.4  Recombinant DNA/RNA Use
    5.4.1   In the laboratory
    5.4.2   Human Gene Transfer (Therapy)
    5.4.3   Transgenic Animals
    5.4.4   Transgenic Plants
    5.5  Restricted Research Activities - 61 KBPDF file*
    5.5.1   Select Agents
    5.5.2   Attenuated Strains of Material on the Select Agents List
    5.5.3   Non-viable Agents on the Select Agents List
    5.5.4   Select Agent Toxin Exclusions
    5.5.5   Select Agents in Clinical or Diagnostic Samples
    5.5.6   Requesting Exemptions from the Select Agent Regulation
    5.5.7   Denial, Revocation, or Suspension of Registration
    5.6  Prohibited Research Activities

    Section 6.    Biosafety Containment Levels

      1. Laboratory Biosafety Level 1 (BSL-1)
      2. Laboratory Biosafety Level 2 (BSL-2)
      3. Laboratory Biosafety Level 3 (BSL-3)
      4. Laboratory Biosafety Level 4 (BSL-4)
      5. Animal Biosafety Level 1 (ABSL-1)
      6. Animal Biosafety Level 2 (ABSL-2)
      7. Animal Biosafety Level 3 (ABSL-3)
      8. Animal Biosafety Level 4 (ABSL-4)

    Section 7.    Biological Agents and Biohazard Classification - 45 KBPDF file*

      1. Risk Group 1 (RG1)
      2. Risk Group 2 (RG2)
      3. Risk Group 3 (RG3)
      4. Risk Group 4 (RG4)
      5. Restricted Animal Pathogens

    Section 8.    Administrative Controls

    8.1  Labels and Signs

      1. Laboratory Safety Plan (Standard Operating Procedures)
      2. Medical Surveillance
      3. Training
      4. Audits & Site Visits
      5. Visitors/Minors in the lab

    Section 9.    Engineering Controls

      1. Biological Safety Cabinets - 48 KBPDF file*
      2. Personal Protective Equipment
      3. Centrifuges
      4. Autoclaves
      5. Other Safety Equipment

    Section 10.  Work Practices

      1. Prudent Practices, Housekeeping, Hygiene
      2. Universal Precautions
      3. Syringes and Needles
      4. Use of Biological Safety Cabinets
      5. Blenders, Ultrasonic Disrupters, and Grinders
      6. Laundry
      7. Decontamination Procedures
      8. Tuberculosis and the Workplace – Respiratory Fit Testing

    Section 11.  Management of Biohazardous Materials - 29 KBPDF file*

      1. Medical Waste Management
      2. Storage
      3. Recordkeeping
      4. Laboratory Security

    Section 12.  Transportation of Biologic & Infectious Substances

      1. Within UAB
      2. Shipments From UAB
      3. Import and Export of Biological Materials
      4. Select Agent Transfer

    Section 13.  Emergency Response - 38 KBPDF file*

      1. Needlesticks and Exposures to Human Blood, Body Fluids, and Tissues
      2. Other Personal Injury
      3. Spill Response
      4. Other Emergencies

    APPENDICES

      1. UAB Biosafety Committee - 10 KBPDF file*
      2. UAB Employee Occupational Health Program - 43 KBPDF file*
      3. Exposure Control Plan Template - 71 KBPDF file*
      4. DOT Guide for Transporting Infectious Substances Safely - 1696 KBPDF file*
      5. Properties of Common Disinfectants
      7. Project Registration
      8. Registration for Recombinant DNA/RNA Research
      9. Select Agent Program Registration
      10. UAB Medical Waste Management Plan - 32 KBPDF file*
      11. Herpesvirus Simiae (Herpes B) Guidelines
      12. Guidelines for Work with Vaccinia Vectors
      13. Guidelines for Work with Adenoviral Vectors


        The full Biosafety Manual in one download - 270 KBPDF file*

*If you have questions about downloading files Click Here.

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