UAB Biosafety Manual

Download the full Biosafety Manual591 KB

Download each section individually below:

Note: only sections of the manual that are currently available are offered for download

TABLE OF CONTENTS 38 KB

1. Introduction 14 KB


2. Program Administration 53 KB
   
   
   1. Office of the Vice President for Financial Affairs
   2. UAB Institutional Biosafety Committee
   3. The Department of Occupational Health & Safety
      1. Biosafety Division
      2. Biosafety Officer
   4. Deans/Department Chairs/Directors
   5. Principal Investigators/Laboratory Directors
   6. Laboratory Staff
   7. Liability Considerations
   8. Incidents of Non-compliance


3. Project Registration
   
   
   1. New Project Registration
   2. Renewals
   3. Modifications of previously registered projects
   4. IBC/Biosafety Approval
   5. Appeal Process


4. Principles of Biosafety 33 KB
   
   
   1. Containment
      1. Primary Containment (Safety Equipment)
      2. Secondary Containment (Facility Design)
   2. Basic Laboratory Safety
   3. Biosafety Levels - in brief


5. Biohazards and Potentially Infectious Materials
   
   
   1. Definitions
   2. Potentially Infectious Materials Use
      1. Microbes
      2. Human Blood, Blood Products, Body Fluids and Tissues
      3. Tissue/Cell Culture
   3. Animal Use
      1. Minimum Biosafety Guidelines
      2. Infected Animals
      3. Use of Non-human Primates
   4. Recombinant DNA/RNA Use
      
      1. In the laboratory
      2. Human Gene Transfer (Therapy)
      3. Transgenic Animals
      4. Transgenic Plants
   5. Restricted Research Activities 61 KB
      1. Select Agents
      2. Attenuated Strains of Material on the Select Agents List
      3. Non-viable Agents on the Select Agents List
      4. Select Agent Toxin Exclusions
      5. Select Agents in Clinical or Diagnostic Samples
      6. Requesting Exemptions from the Select Agent Regulation
      7. Denial, Revocation, or Suspension of Registration
   6. Prohibited Research Activities


6. Biosafety Containment Levels
   
   
   1. Laboratory Biosafety Level 1 (BSL-1)
   2. Laboratory Biosafety Level 2 (BSL-2)
   3. Laboratory Biosafety Level 3 (BSL-3)
   4. Laboratory Biosafety Level 4 (BSL-4)
   5. Animal Biosafety Level 1 (ABSL-1)
   6. Animal Biosafety Level 2 (ABSL-2)
   7. Animal Biosafety Level 3 (ABSL-3)
   8. Animal Biosafety Level 4 (ABSL-4)


7. Biological Agents and Biohazard Classification 45 KB
   
   
   1. Risk Group 1 (RG1)
   2. Risk Group 2 (RG2)
   3. Risk Group 3 (RG3)
   4. Risk Group 4 (RG4)
   5. Restricted Animal Pathogens


8. Administrative Controls
   
   
   1. Labels and Signs
   2. Laboratory Safety Plan (Standard Operating Procedures)
   3. Medical Surveillance
   4. Training
   5. Audits & Site Visits
   6. Visitors/Minors in the lab


9. Engineering Controls
   
   
   1. Biological Safety Cabinets 48 KB
   2. Personal Protective Equipment
   3. Centrifuges
   4. Autoclaves
   5. Other Safety Equipment


10. Work Practices
    
    
    1. Prudent Practices, Housekeeping, Hygiene
    2. Universal Precautions
    3. Syringes and Needles
    4. Use of Biological Safety Cabinets
    5. Blenders, Ultrasonic Disrupters, and Grinders
    6. Laundry
    7. Decontamination Procedures
    8. Tuberculosis and the Workplace - Respiratory Fit Testing


11. Management of Biohazardous Materials

    [Under construction]

12. Transportation of Biologic & Infectious Substances
    
    
    1. Within UAB
    2. Shipments From UAB
    3. Import and Export of Biological Materials
    4. Select Agent Transfer


13. Emergency Response 38 KB
    
    
    1. Needlesticks and Exposures to Human Blood, Body Fluids, and Tissues
    2. Other Personal Injury
    3. Spill Response
    4. Other Emergencies

APPENDICES

1. UAB IBC Roles and Responsibilities 41 KB
2. UAB Employee Occupational Health Program 43 KB
3. Exposure Control Plan Template 71 KB
5. Properties of Common Disinfectants
7. Project Registration 201 KB
8. Registration for Recombinant DNA/RNA Research 151 KB
9. Select Agent Program Registration
10. UAB Medical Waste Management Plan 38 KB
11. Herpesvirus Simiae (Herpes B) Guidelines
12. Immunization Policy & Biosafety Considerations for Users of Vaccinia, Vaccinia Vectors & Other Orthopox Viruses 93 KB
13. Guidelines for Work with Adenoviral Vectors

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